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    Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications

    • Research article
    • Open access
    • Published:

    BMC Biotechnologyvolume 7, Article number: 76 () Cite this article

    • 33k Accesses

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    Abstract

    Background

    EvaGreen (EG) is a newly developed DNA-binding dye that has recently been used in quantitative real-time PCR (qPCR), post-PCR DNA melt curve analysis and several other applications. However, very little is known about the physicochemical properties of the dye and their relevance to the applications, particularly to qPCR and post PCR DNA melt curve analysis. In this paper, we characterized EG along with a widely used qPCR dye, SYBR Green I (SG), for their DNA-binding properties and stability, and compared their performance in qPCR under a variety of conditions.

    Results

    This study systematically compared the DNA binding profiles of the two dyes under different condition

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    Bond girl Eva Green says should always be played by a man

    LOS ANGELES: Former Bond Girl Eva Greenis not in the favour of flipping the gender of spy as she believes it would be wrong to ignore the history of the character, which has always been played by a man.

    The "Casino Royale" star, considered one of the best Bond girls in the history of the franchise, says women should create their own action film legacy.

    Daniel Craig is set to leave the franchise after the release of Cary Fukunaga's "Bond 25" in April and there is already a lot of speculation about his replacement with names such as Idris Elba and Richard Madden doing the rounds.

    Some actors and fans are in favour of giving a gender swap to the character as they believe it is time for a female actor to take on the role, created by Ian Fleming.

    Agencies

    A still from the movie 'Casino Royale'. (Image: IMDb)



    "I'm for women, but I really think James Bondshould remain a man. It doesn't make sense for him to be a woman. W
  • eva green biography height predictor

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    Eva Green: Astrological Article and Chart

    You will find below the horoscope of Eva Green with her interactive chart, an excerpt of her astrological portrait and her planetary dominants.

    		Born:Sunday, July 6, , AM
    In:Paris 14e (France)
    Sun: 14°25' CancerAS: 4°58' Virgo
    Moon:28°17' AriesMC: 27°49' Taurus
    Dominants: Virgo, Cancer, Aries
    Jupiter, Mercury, Uranus
    Houses 11, 1, 2 / Earth, vatten / Mutable
    Chinese Astrology: Metal Monkey
    Numerology: Birth Path 4
    Height: Eva Green is 5' 7" (1m70) tall
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    Additional information on the source of the birth time is sometimes available in the biography excerpt below.

    Horoscopes having the same Big Three (Sun in Cancer, Moon in Aries, Ascendant in Virgo) : Henry VIII of England, Joy Green, Camille Grimaldi, Pierre Laval, Titouan Lamazou, Paul Ricard, Christophe Michalak, Olivier Adam, Alain Lambert List of all the celebrities having the same Big Three.

    Horoscopes havin